Composite

Part:BBa_K4577008:Design

Designed by: Ma Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-06)


AmoA gene with the tac promotor, the strong bacterial RBS and the lambda t0 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


Design Notes

The codons have been optimised for the Sinorhizobium meliloti Sm1021 strain.

References

[1] Szabolcs Semsey, Sandeep Krishna. "The effect of LacI autoregulation on the performance of the lactose utilization system in Escherichia coli" Nucleic Acids Res 2013 Jul; 41(13): 6381–6390

[2] Schwaiger KN, Voit A, Dobiašová H, Luley C, Wiltschi B, Nidetzky B. Plasmid Design for Tunable Two-Enzyme Co-Expression Promotes Whole-Cell Production of Cellobiose. Biotechnol J. 2020 Nov;15(11):e2000063. doi: 10.1002/biot.202000063. Epub 2020 Aug 7. PMID: 32668097.

[3] Elowitz MB, Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature. 2000 Jan 20;403(6767):335-8. doi: 10.1038/35002125. PMID: 10659856.